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Suspension Cell Culture
Home > Technology > Bioreactor > Suspension Cell Culture > Stainless Steel Suspension Cell Culture Bioreactor
  • Stainless Steel Suspension Cell Culture Bioreactor
  • Stainless Steel Suspension Cell Culture Bioreactor
  • Stainless Steel Suspension Cell Culture Bioreactor
  • Stainless Steel Suspension Cell Culture Bioreactor
  • Stainless Steel Suspension Cell Culture Bioreactor

Stainless Steel Suspension Cell Culture Bioreactor



Duoning provides customized bioreactors with volume of >20 L for suspension cell culture, as well as supporting systems such as liquid mixing, inactivation and emulsification. In addition, we can provide customers with process design, pipeline construction, system integration, bioreactor design and manufacturing, control software development and other services.

 

System features

?   These systems can be used for suspension culture, microcarrier culture for monoclonal antibody, vaccine or protein-based therapeutics production, to achieve multiple use in one tank.

?   Suspension and microcarrier culture using propulsion impeller or lifting impeller, low shear force, short mixing time, good mixing effect

?   Deep microbubble direct air supply/bubble-free air supply device provide good oxygen transfer, meets the requirements of high-density cell culture, and reduces the damage to cells

?   Using jacket and tank dual temperature PID control, temperature control is stable and accurate

?   Sterilization-in-place sampling/sampling valve group to ensure safe, reliable and convenient operation

?   Air intake/exhaust filter sterilization can be carried out independently to meet the requirements of filter replacement during the culture process

?   CO2/O2/AIR flow rate adopts thermal mass flow controller, to realize the automatic feedback control of gas flow rate

?   Dissolved oxygen control can adopt AIR/O2 or AIR/O2/N2 mode. The DO control adopts unique PID control logic for stable and accurate control

?   Deep sparger and overlay air supply adopt different control methods in sterilization process, to ensure thorough sterilization and no dead end

?   Deep sparger uses a combination of hardware and software, effectively eliminate culture fluid reflux

?   PH control adopts time judgment to avoid the simultaneous addition of CO2 and base

?   Peristaltic pump control allows external mobile peristaltic pump, easy to replace

?   Pipeline and frame adopt open design, easy to use and maintain

?   Pipeline adopts 3A standard, comply with GMP and BPE requirements

?   Connection seal of the mixing impeller in the tank effectively avoids dead end and is easy to clean

?   Pipeline construction is carried out in cleanroom to ensure welding quality

?   Material inlet and tank bottom valves can be sterilized separately

?   All the pipelines and tee connections on the tank body meet 3D requirements

?   Dual mechanical seal, sterilization-in-place; or bottom magnetic stirring

?   The control system adopts PLC control

?   Servo motor, large torque, realizes stable and reliable work

?   Powerful software functions, such as parameter setting, display control, test parameter, curve analysis, data storage, and output printing

?   System adopts electronic signature, which meets the requirements of GMP audit

 

Technical parameters

Tank body

Total volume

40 L

75 L

150 L

Working volume

30 L

50 L

120 L

Diameter-height   ratio

1:1.2

1:1.2

1:1.2

Material

316L SS

Polishing rate

Internal: 0.2-0.4 μm; external: 0.8μm

Manufacturing   standard

ASME, PED, GB D1/D2, ISO9001:2000


Material 316L stainless steel, jacket 304 stainless steel

Polishing accuracy of the surface in contact with the material Ra≤0.4

Design pressure: 0.3 MPa, working pressure: 0.25 MPa

Connection configuration: safety glass window, side view window, feed valve group port, foam sensor port, pressure sensor port, in-place sampling port, air intake port, bottom valve, inoculation valve group port, base feed port, safety bursting disc

 

Stir

Type

Top/bottom stir, Dual mechanical or magnetic drive, PID control, clean air lubrication (mechanical seal)

Display

Digital display, ±1 rpm

Stir speed

25~250 rpm, ±1 rpm

Impeller

Plow type impeller

Detection probe

pH

2~12, PID control, accuracy: ±0.01, correlate with base and CO2

DO

0~200%, PID control, accuracy: ±1%, correlate with O2, Air

Liquid level

SS probe, PID control

Temperature

Pt100, PID Control, Digital display, ±0.1℃

Gas control

Flowrate measurement

Thermal mass flow controller (optional)

Gas

Four-gas control   system (N2, O2, CO2, Air) , solenoid valve control

Intake filter

0.2 μm, PTFE

Exhaust filter

0.2 μm, PTFE

Exhaust condenser

316L SS

Pressure regulation

Automatic pressure regulating system, including pressure sensor, and regulating valve

Liquid feed

Two peristaltic pump

Variable speed pump, and flow is accumulated: regulate pump speed according to control requirement, thus control flow rate.

Temperature

Control method

PID, Automatic sterilization; The temperature is controlled by the external heater and the cooling water under the control of the control system

Control accuracy

Display accuracy: 0.1℃, control accuracy: ±0.1℃

Control range

5 ℃~65 ℃ above cooling water temperature

Control system


Industrial programmable logic controller

Optional configurations


Weighing, tank lid automatic lifting



AC380V two phase five wire, 15Kw

Water, electricity, gas requirements


Cooling water pressure: 0.15 MPa

Cooling water temperature: not higher than 30 ℃

Input air pressure: 0.2 MPa (subject to drying and pre-filtration)

Input steam pressure: 0.18 MPa (must be pre-filtered)

For more information about custom systems, please contact your local representatives or technical support team of Duoning.

 



 


Contact Us
  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
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