His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.
His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.
His-tag protein purification resin (GP-IDA) uses super-macroporous polyacrylate beads as matrix, iminodiacetic acid (IDA) as chelating ligand, and conjugates Ni2+. This product features low mass transfer resistance and is suitable for capturing His-protein from high viscosity feed, including supernatant of E. coli cell lysis.
His-tag protein purification resin (CP-NTA) uses macroporous polyacrylate beads as matrix and nitrilotriacetic acid (NTA) as chelating ligand, which is resistant to low concentration of reducing agent added in protein solution, including B-mercaptoethanol or dithiothreitol (DTT).
His-tag protein purification resin (HP-Plus) uses polyacrylate beads as matrix and adopts unique metal chelating ligand (Plus) from Bogen (A Duoning company), which can stably adsorb Ni2+ without falling off even in solutions containing EDTA; and it can be CIP with NaOH while no need to be re-conjugate Ni2+. This product can greatly save time of sample pretreatment and after-process treatment of resin. It is especially suitable for purification of His-tag proteins expressed and secreted eukaryotic cells, including insect and mammalian cells.
Product features
Specifications | His-tag protein purification resin （GP-IDA） | His-tag protein purification resin （GP-NTA） | His-tag protein purification resin （HP-Plus） |
Matrix | Super macroporous polyacrylate hard gel | Macroporous polyacrylate hard gel | Polyacrylate hard gel |
Ligand | IDA | NTA | Plus |
Average particle size | 70 μm | 70 μm | 70 μm |
Reducing agent resistance | NO | β-Me≤100 mM DTT≤5mM | EDTA≤50mM |
Dynamic binding capacity | ≥ 40 mg/ml | ≥ 30 mg/ml | ≥ 10 mg/ml |
Max flow rate | 1000 cm/hr | 1000 cm/hr | 1000 cm/hr |
Max pressure | 1 MPa | 1 MPa | 1 MPa |
Storage | 4-30 ℃（20% ethanol） | 4-30 ℃（20% ethanol） | 4-30 ℃（20% ethanol） |
Features | High protein capacity | Low metal ion leaching rate | Resistant to high concentration of reducing agents |
For different purification requirements, Bogen also provides His-tag protein purification resins based on agarose soft gel matrix. Considering different His protein binding ability and transition metal ion selectivity, we also provide IMAC products without chelated metal ions, users can choose to chelate Cu2+, Zn2+, Co2+ and other transition metal ions.
Specifications | His-tag protein purification resin （NTA） | His-tag protein purification resin （IMAC） |
Matrix | Agarose soft gel | Agarose soft gel |
Ligand | NTA | IDA/NTA/Plus |
Average particle size | 90 μm | 90 μm |
Max flow rate | 500 cm/hr | 500 cm/hr |
Max pressure | 0.3 MPa | 0.3 MPa |
Storage | 4-8 ℃（20% ethanol） | 4-8 ℃（20% ethanol） |
Features | Low metal ion leaching rate | The type of metal ion can be freely selected |
Case Study
Purifying His-fusion protein from supernatant of E.Coli lysis.
Column size 1.6cm ID x 2.5cm
Buffer: Buffer A: 20 mM PB+0.15M NaCI, pH6.0 (wash1)；Buffer B: Buffer A+10mM imidazole (wash2); Buffer C: Buffer A+100mM imidazole (elute)
Flow rate: 5 ml/min
Detection UV wavelength: 280 nm
Feed: Crude cell lysate 520 ml
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