MixQ virus purification resin is mixed mode resin developed by Bogen (A Duoning Company) for isolation and purification of virus-based products. This product features a core-shell structure. The shell is hydrophilic polymer with defined pore size, due to large size, the virus particles flow through under exclusion effect, while the small impurities pass through the shell and enter the core. The core is polymethacrylate with rigid structure, together with n-octylamine as ligand, which absorbs impurities through ion exchange/hydrophobic interaction, thus separating viruses and impurities.
We provide two types of MixQ virus purification resins: MixQ700 (>MW 700,000) and MixQ400 (>MW 400,000), based on the molecular weight of size exclusion.
Product Features
? Mode of virus flow-through, impurity been adsorbed
? Impurity proteins can also be adsorbed at high salt concentrations without buffer exchange
? The composition of the virus flow-through solution is same as feed solution, maintaining virus activity
Product Specification
Parameter | Specification | |
Product Name | MixQ700S virus purification resin | MixQ700L virus purification resin |
Average particle size | 70 μm | 120 μm |
Matrix | Polyacrylate hard gel | |
Size exclusion molecular weight | 700 KDa | |
Max Pressure | 1 MPa | |
Dynamic capacity | ≥10mg/mL | |
PH Stability | Resistant to 0.5 M NaOH + 30% IPA cleaning | |
Storage | 4-30 ℃ (20% ethanol) or 5-10 mM NaOH | |
Parameter | Specification | |
Product Name | MixQ400S virus purification resin | MixQ400L virus purification resin |
Average particle size | 70μm | 120 μm |
Matrix | Polyacrylate hard gel | |
Size exclusion molecular weight | 400 KDa | |
Max Pressure | 1MPa | |
Dynamic capacity | ≥10mg/mL | |
PH stability | Resistant to 0.5 M NaOH + 30% IPA cleaning | |
Storage | 4-30 ℃ (20% ethanol) or 5-10 mM NaOH | |
Case Study 1
Chromogram of vaccine antigen purification process by MixQ700Lvirus purification resin
Column size: 1.6cm ID × 10cm
Process preparation: concentrate 15 X
Chromatography process parameters:
Flowrate: 2 mL/min
Loading volume: 20 mL
Collect flow-through fraction
Sample | Volume | Virus titer (TCID50/0.1mL) | A280-IGG (μg/mL) |
Concentrated original sample | 30 mL | 7.8 | 21026 |
Collected purified sample | 40 mL | 7.8 | 4042 |
Electrophoresis results of virus purification process by MixQ mixed mode resin
Case Study 2
Adenovirus vector purification
Resin: MixQ700L virus purification resin
Column size: 1mL
Flowrate: 1mL/min
Sampe: Model vaccine antigen
MixQ Mixed Mode Resins - for virus purification
Product Name | Part No. | Specification | Product Name | Part No. | Specification |
MixQ700L virus purification resin | BG65-1010-02 | 50mL | MixQ700S virus purification resin | BG65-1110-02 | 50mL |
BG65-1010-04 | 500mL | BG65-1110-04 | 500mL | ||
BG65-1010-05 | 1L | BG65-1110-05 | 1L | ||
BG65-1010-07 | 10L | BG65-1110-07 | 10L | ||
BG65-1010-08 | 20L | BG65-1110-08 | 20L | ||
MixQ400L virus purification resin | BG65-2010-02 | 50mL | MixQ400S virus purification resin | BG65-2110-02 | 50mL |
BG65-2010-04 | 500mL | BG65-2110-04 | 500mL | ||
BG65-2010-05 | 1L | BG65-2110-05 | 1L | ||
BG65-2010-07 | 10L | BG65-2110-07 | 10L | ||
BG65-2010-08 | 20L | BG65-2110-08 | 20L |
MixQ Mixed Mode Resin Column - for virus purification
Product Name | Specification | Size exclusion molecular weight | Capacity | Max Pressure | Max Flow rate | Application/Feature |
MixQ700L virus purification column | 5mL | 700KDa | ≥10mg/mL | 1MPa | 1000cm/h | Scale-up study of virus purification process |
10mL | ||||||
MixQ700S virus purification column | 5mL | 700KDa | ≥10mg/mL | 1MPa | 1000cm/h | |
10mL | ||||||
MixQ400L virus purification column | 5mL | 400KDa | ≥10mg/mL | 1MPa | 1000cm/h | |
10mL | ||||||
MixQ400S virus purification column | 5mL | 400KDa | ≥10mg/mL | 1MPa | 1000cm/h | |
10mL |
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